mrna oligo binding buffer (InDevR Inc)
Structured Review

Mrna Oligo Binding Buffer, supplied by InDevR Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mrna oligo binding buffer/product/InDevR Inc
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Analytical Performance of a Multiplexed Microarray Assay for Rapid Identification and Quantification of a Multivalent mRNA Vaccine"
Article Title: Analytical Performance of a Multiplexed Microarray Assay for Rapid Identification and Quantification of a Multivalent mRNA Vaccine
Journal: Vaccines
doi: 10.3390/vaccines12101144
Figure Legend Snippet: ( a ) Schematic of each mRNA construct with naming and binding locations of the capture oligos. Performance results for blue-shaded capture oligos are the focus of this work. ( b ) Microarray layout where each capture oligo was printed in triplicate in three locations on the microarray (n = 9 spots). ( c ) Schematic of the assay detection principle, in which each printed capture oligo can bind an mRNA construct in a sequence-specific manner and be fluorescently labeled via the polyA tail. ( d ) Representative fluorescence images of each monovalent encapsulated mRNA construct captured and labeled on the microarray, as well as a quadrivalent mixture of the four mRNA constructs, each at a sample concentration of 20 µg/mL. The relevant capture oligos are highlighted for each monovalent sample, with brighter green indicating a higher fluorescence signal.
Techniques Used: Construct, Binding Assay, Microarray, Sequencing, Labeling, Fluorescence, Concentration Assay
Figure Legend Snippet: Reactivity and specificity of monovalent mRNA constructs encapsulated in LNPs at 5 µg/mL.
Techniques Used: Construct
Figure Legend Snippet: Analytical sensitivity and range for mRNA encapsulated in LNPs.
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Figure Legend Snippet: Precision and accuracy of mRNA encapsulated in LNPs.
Techniques Used:
Figure Legend Snippet: Reactivity and specificity of naked mRNA at 20 µg/mL.
Techniques Used:
Figure Legend Snippet: ( a – d ) Representative calibration curves from a quadrivalent mixture of naked mRNA are shown for each capture oligo: ( a – c ) were fit to a quadratic regression with R 2 ≥ 0.99, and ( d ) D(i) was fit to a 4PL regression with R 2 ≥ 0.99.
Techniques Used:
Figure Legend Snippet: Precision and accuracy (naked and unencapsulated mRNA).
Techniques Used: